Puresyn, Inc.

Malvern,  PA 
United States
http://www.puresyn.com


Puresyn - manufacturing services producing high quality DNA

Puresyn, Inc. is focused on and dedicated to providing manufacturing services capable of producing high quality DNA which can be used for many applications including GMP manufacture of gene therapy products.  These services include the following:

  • Contract Plasmid DNA Production Services
    • TransfectionReady
    • ResearchReady
    • INDReady®

Brands: Contract Plasmid DNA Production Services - TransfectionReady, ResearchReady, INDReady®


 Products

  • INDReady ® Plasmid DNA
    Our INDReady® Program is designed to meet the requirements for cGMP intermediates. INDReady® Plasmid DNA is produced so that all processing components used are dedicated for single plasmid use or are disposable....

  • All components used are animal free or are treated in compliance with appropriate regulations to reduce the risk of TSE/BSE contamination.  These contracts have a dedicated scientist and are processed in dedicated rooms.  The processing utilizes our proprietary resin, PolyFlo® (DMF13090) and results in highly pure DNA meeting the specifications. A Manufacturing Summary of all documentation can be provided and is suitable for IND submission.

    Custom Premium INDReady® Plasmid DNA

    PARAMETER SPECIFICATION
    Appearance Clear, Colorless, No Visible Particulates
    Sterile Fill Sterile Filled by Quality
    A260:280 1.7-2.0
    Concentration Amount Ordered (mg) / 10ml
    DNA Homogeneity ≥90%
    Residual RNA None Detected @ 1.0ug load
    ssDNA, Linear DNA, Chromosomal DNA None Detected @ 1.0ug load
    Protein Reported
    pH of Final Product 7.5-8.5
    Endotoxin <30 EU/mg
    Identity Consistent with Provided Information for 2 Restriction Reactions
    Five Day Bioburden No growth on plates
    Bottling Collaborator Defined
  • ResearchReady Plasmid DNA
    Service utilizes our proprietary resin, PolyFlo® (DMF13090) and results in highly pure DNA meeting the specifications detailed below. ResearchReady Plasmid DNA is suitable for a variety of research applications....

  • There are three levels of guaranteed specifications available each with customizable options that can be included as needed:  Basic, Intermediate, and Premium.

    PARAMETER

    BASIC

    INTERMEDIATE

    PREMIUM

    Sterile Fill

    Sterile Fill

    Sterile Fill

    Sterile Fill

    Appearance

    Clear, Colorless, No Visible Particulates

    Clear, Colorless, No Visible Particulates

    Clear, Colorless, No Visible Particulates

    A260:280

    1.7-2.0

    1.7-2.0

    1.7-2.0

    Concentration

    ³Amount Ordered (mg) ¸ 10ml

    ³Amount Ordered (mg) ¸ 10ml

    ³Amount Ordered (mg) ¸ 10ml

    DNA Homogeneity

    ³80% All Supercoiled Forms

    ³85% All Supercoiled Forms

    ³90% All Supercoiled Forms

    Residual RNA

    None Detected @ 0.2mg load

    None Detected @ 0.2mg load

    None Detected @ 1.0mg load

    Residual ssDNA

    Not Tested

    None Detected @ 0.2mg load

    None Detected @ 1.0mg load

    Residual Linear DNA

    Not Tested

    None Detected @ 0.2mg load

    None Detected @ 1.0mg load

    Residual Chromosomal DNA

    Not Tested

    None Detected @ 0.2mg load

    None Detected @ 1.0mg load

    Protein

    Not Tested

    Not Tested

    Reported

    Endotoxin

    Not Tested

    <100 EU/mg

    <30 EU/mg

    Identity

    Not Tested

    Not Tested

    Consistent with Provided Information for 2 Restriction Reactions

     Additional Services

    • Transformation
    • Specific Range for Concentration – would you like to request 1.0 – 1.1mg/ml (0.95-1.14mg/ml) or similar?
    • Aliquoting – how many mg per aliquot would you like to request?
    • Restriction ID (two included with Premium only)
    • Overnight OR Five Day Bioburden test
  • TransfectionReady Plasmid DNA
    Service which is a convenient and cost effective replacement for hours of research time lost performing in-house purification kits....

  • We have two scales: Maxi- up to 1mg of Plasmid DNA for $99 and Mega- up to 4mg of Plasmid DNA for $200.  The process is simple. Once we receive your order and plasmids the turnaround time is 5 business days. We ship by the 6th day or sooner if our schedule as our schedule allows.  At shipment, we will report 260:280, concentration (mg/ml), volume and quantity for each plasmid. There are no guaranteed specifications and results are dependent on plasmid yield.