Sartorius

http://www.sartorius.com

Advancing Bioprocessing in Cell and Gene Therapy

Octet^®BLI Label-Free Detection Systems
Based on Bio-Layer Interferometry (BLI), Octet^® BLI systems enable real-time, label-free analysis for the determination of kinetics, affinity and antibody/protein quantitation. This fluidics-free instrument platform offers several advantages over other label-free techniques. The platform has become an invaluable technique analyzing therapeutic biomolecules at various stages of discovery, development, manufacturing and quality control.

New! Octet^®AAVX Biosensors
Rapid quantitation of adeno-associated viral (AAV) capsids for various serotypes used in bioprocess development and quality control in gene therapy. AAV titer can be measured on the Octet^® platform in as little as 15 minutes, providing an efficient alternative to time-consuming and laborious techniques such as ELISA and HPLC.

Growth Factors and Cytokines
Sartorius offers a large variety of growth factors and cytokines for ex vivo cell culture. These raw and ancillary materials are widely used in the field of cell and gene therapy as well as regenerative medicine.

CellGenix^®Serum-free Media
Optimized serum-free media solutions for ex vivo cell expansion and differentiation of various human cell types. Since different cells require different cell culture conditions, we offer multiple optimized media solutions.

High Quality – High Performance, from Research to ATMP
CellGenix^® serum-free GMP media combine a maximum of quality and safety with excellent performance due to state-of-the-art production, stringent quality control and expert regulatory support. This ensures reliable and consistent results which is crucial for the standardization of your ATMP manufacturing process.

Brands: Octet® Bio-Layer Interferometry Platform and CellGenix® Quality GMP Reagents

Videos

Show Specials

Tools and Technology Presentation: Rapid AAV Titer for Bioprocessing in Gene Therapy Using Octet® BLI Platform (Mar 13, 2023)

Friday May 19
5:15 pm

Products

Octet® R8 Bio-Layer Interferometry System

High Throughput System for Biologics Discovery, Development, QC, and Manufacturing... More Info Less Info

The Octet^® R8 label-free protein analysis system provides fast, high throughput, and accurate characterization of biomolecules and is compatible with longer experimental run times and seamless integration into GxP-compliant labs during biologics development.

The 8-channel Octet^® R8 system performs quantitation and kinetic analysis of up to 96 samples in 30 minutes to 2.5 hours, depending on the specific assay. An optional microplate evaporation cover minimizes losses in sample volume, allowing post-analysis sample recovery even after an experiment.

The system can be used for a wide range of analyses including kinetic analysis, titer of IgG and other proteins, reagent qualification, immunoassay development, bioprocess development, quality analysis, crude antibody screening, epitope binning/mapping, ligand binding assays, small molecule analysis, elucidating cell signaling mechanisms, and infectious disease monitoring. Analysis can be done using a single channel or up to eight channels, enabling flexibility in sample throughput.

Octet® AAVX Biosensors

Rapid Quantitation of AAV Capsids for Various Serotypes for Bioprocess Development and Quality Control in Gene Therapy... More Info Less Info

Rapid Quantitation of AAV Capsids for Various Serotypes for Bioprocess Development and Quality Control in Gene Therapy.

Rapid, direct, and label-free quantitation of AAV capsids in crude and purified samples
Specificity to various AAV serotypes, including AAV1 – AAV9 and AAVrh10
Direct quantitation of AAV capsids in the dynamic range of 8.5E8 – 1.0E13 vp/mL
Efficient and cost-effective regeneration for biosensor reuse up to 10 - 20 times
Good correlation of the results with ELISA

AAV vector development and production at industrial scale requires an efficient method for accurate quantitation of viral capsids in both upstream and downstream bioprocessing. Octet^® Bio-Layer Interferometry (BLI) systems in combination with the Octet^® AAVX Biosensors offer a rapid, label-free, and high-throughput method for quantification of AAV capsids at various stages in bioprocessing.

Octet^® AAVX Biosensors are pre-immobilize with Thermo Scientific^TM CaptureSelect^TM Biotin Anti-AAVX Conjugate, from Thermo Fisher Scientific Inc, that binds with high affinity and specificity to various native and recombinant AAV serotypes, including AAV1 to AAV9 and AAVrh10. Due to its high specificity the AAVX biosensor can be used to quantitate AAV capsids in both purified and crude cell culture samples with titer in the range of 8.5E8 – 1.0E13 vp/mL for most serotypes.

AAV titer can be measured on the Octet^® platform in as little as 15 minutes, providing an efficient alternative to time-consuming and laborious techniques such as ELISA and HPLC. Finally, the Octet^® AAVX Biosensors can be efficiently regenerated up to 20 times, which makes them an extremely useful and cost-effective solution for high-throughput AAV capsid titer measurement in process development and QC.