Combining characteristics of reduced size and the RNA-OUT non-antibiotic resistance selection marker, the platform is suited for a range of therapeutic applications. Aldevron can provide customized Nanoplasmid DNA for each client. Nanoplasmid advantages include:
- High Transgene Expression
- Increased Manufacturing Yields
- Improved Safety Profile by Eliminating Antibiotic Resistant Gene
- Save Time and Cost-Effective
Antibiotic-free selection provided by RNA-OUT
The RNA-OUT platform works by repressing the expression of a counter-selectable marker (SacB) from the host chromosome. SacB encodes for the levansucrase enzyme, which creates a toxic environment in the presence of sucrose, which leads to cell death. Transforming the Antibiotic-free Nanoplasmid in the host expresses a 150bp RNA-OUT antisense RNA (ROUT) that binds the SacB mRNA and represses levansucrase expression, prevents toxicity and to maintain cell survival. The RNA-OUT selectable marker can be used to retrofit existing antibiotic resistance DNA plasmids into antibiotic-free vectors counter-selection system.
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