Walter E. Washington Convention Center
Washington, D.C.
May 16-19, 2022

Integrated DNA Technologies (IDT)  

Coralville,  IA 
United States
http://www.idtdna.com
  • Booth: 166

Integrated DNA Technologies, Inc. (IDT) develops, manufactures, and markets nucleic acid products for the life sciences industry in the areas of academic and commercial research, agriculture, medical diagnostics, and pharmaceutical development. IDT has developed proprietary technologies for genomics applications such as next generation sequencing, CRISPR genome editing, synthetic biology, digital PCR, and RNA interference. Through its GMP services, IDT manufactures products used by scientists researching many forms of cancer and most inherited and infectious diseases. IDT is widely recognized as the industry leader in custom nucleic acid manufacture, serving over 130,000 life sciences researchers. IDT was founded in 1987 and has its manufacturing headquarters in Coralville, Iowa, USA, with additional manufacturing sites in San Diego, California, USA; Research Triangle Park, North Carolina, USA; Ann Arbor, Michigan, USA; Leuven, Belgium; and Singapore. For more information, please visit www.idtdna.com and follow on Twitter, LinkedIn, Facebook, YouTube, and Instagram.

Brands: See how IDT has developed proprietary technologies for creating best-in-class genomics solutions for CRISPR genome editing on our website www.idtdna.com.


 Show Specials


 Press Releases

  • Alt-RHDR Donor Blocks cater to market demand for better homology-directed repair research solutions

    CORALVILLE, Iowa — Global genomics solutions provider Integrated DNA Technologies (IDT) is empowering researchers to drive scientific discoveries in gene editing with the release of Alt-R HDR Donor Blocks, an improved solution for increasing homology-directed repair (HDR) rates in large fragment knock-in experiments. IDT’s Alt-R HDR Donor Blocks incorporate advanced chemical modifications to boost the HDR rates of large genomic changes and insertions, and significantly reduce the occurrence of non-homologous (blunt) integration of the donor sequence. Orders can be placed at www.idtdna.com/hdrdonorblocks

    “It is well established that a key challenge in precise genome editing research is the low efficiency of HDR rates, and our new solution is singularly focused on helping researchers make HDR experiments more efficient and more likely to succeed,” said Mark Behlke, IDT’s Chief Scientific Officer. “Alt-R HDR Donor Blocks are the latest examples of products resulting from extensive wet-bench research conducted by IDT’s R&D scientists. We hope this new product can help increase research yields for the scientific community and serve as a gateway to repairing disease-causing mutations, altering genomes, and enabling more discoveries.”

    In a study, IDT investigated the impact on HDR efficiencies when inserting a green florescent protein tag using long, single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) templates. The outcomes demonstrated that the use of

    Alt-R HDR Donor Blocks resulted in the highest HDR rates compared to unmodified dsDNA or long ssDNA. The ability of Alt-R Donor Blocks to reduce the occurrence of unwanted off-target integrations was also investigated. In addition, results showed a 65% reduction in blunt insertions at a mock off-target double-strand break compared to unmodified dsDNA. When combined with IDT’s Alt-R HDR Enhancer v2, modified Alt-R Donor Blocks exhibited the highest HDR rates at multiple genomic loci and in multiple cell lines tested, and reduced the off-target integration, lowering blunt insertion levels to 1%.

    Ann Morris, PhD, an associate professor at the University of Kentucky, added, “We worked with IDT on a project to generate knock-in zebrafish carrying an in-frame epitope tag in our gene of interest. In-frame knock-ins are notoriously difficult to obtain in zebrafish, but IDT helped us design several candidate donor templates with various modifications to determine which one would work best. The IDT Alt-R HDR Design Tool was easy to work with, and we were able to rapidly test several permutations of HDR donor blocks to successfully obtain our desired knock-in line. IDT staff gave us a lot of helpful advice, and were readily available throughout the process to answer our questions and get feedback on what worked best. We will definitely work with them again on our next knock-in project.”

    IDT’s Alt-R HDR Donor Blocks are available from 201 to 3,000 bases in length, are generated from clonally purified DNA, and utilize the same high-fidelity process as IDT gBlocks™ HiFi Gene Fragments. Benefits include:

    • Modifications that increase successful HDR events
    • Lower unintended blunt integrations at on- and off-target sites
    • Sequence verification by next generation sequencing
    • Higher HDR rates when combined with the Alt-R HDR Enhancer v2

    “Successful CRISPR-mediated knock-in has been a challenge, but I have had great success with IDT’s Alt-R HDR Enhancer v2 and Alt-R HDR Donor Blocks,” said Ravi Nath, a postdoctoral scholar in genetics at Stanford University. “They were critical for generating key lines for my experiments.”

    Alt-R HDR Donor Blocks are the latest addition to IDT’s complete portfolio of Alt-R™ CRISPR genome editing solutions and are designed to meet the evolving needs of researchers who are continuing to push the field of genome editing forward. Earlier this year, IDT launched the rhAmpSeq™ CRISPR Analysis System, an end-to-end solution for characterizing and quantifying the full array of on- and off-target genome editing events in CRISPR research products. IDT’s Alt-R CRISPR portfolio is comprised of solutions to meet researchers’ complete workflow — from design to analysis — to enable research projects through quality, simplicity, and cost efficiency.

    About IDT

    Integrated DNA Technologies, Inc. (IDT) develops, manufactures, and markets nucleic acid products for the life sciences industry in the areas of academic and commercial research, agriculture, medical diagnostics, and pharmaceutical development. IDT has developed proprietary technologies for genomics applications such as next generation sequencing, CRISPR genome editing, synthetic biology, digital PCR, and RNA interference. Through its GMP services, IDT manufactures products used by scientists researching many forms of cancer and most inherited and infectious diseases. IDT is widely recognized as the industry leader in custom nucleic acid manufacture, serving over 130,000 life sciences researchers. IDT was founded in 1987 and has its manufacturing headquarters in Coralville, Iowa, USA, with additional manufacturing sites in San Diego, California, USA; Research Triangle Park, North Carolina, USA; Ann Arbor, Michigan, USA; Leuven, Belgium; and Singapore. For more information, please visit www.idtdna.com and follow on Twitter, LinkedIn, Facebook, YouTube, and Instagram.


 Products

  • Alt-R™ CIRPSR Genome Editing Systems
    The Alt-R CRISPR Systems were developed through comprehensive research on each component of the CRISPR-driven, double-stranded break generation critical for gene disruption and DNA insertion by homologous recombination....

  • Alt-R CRISPR-Cas9 System

    The Alt-R™ CRISPR-Cas9 System includes all of the reagents needed for successful genome editing in your research applications based on the natural S. pyogenes CRISPR-Cas9 system.

    • Benefit from the latest improvements in on- and off-target design and chemical modifications, as well as easy ordering of custom or predesigned guide RNAs.
    • Get optimal editing with high on-target potency and reduced off-target activity with Alt-R HiFi CRISPR-Cas9 nuclease
    • Precisely control editing with efficient delivery of the RNP by lipofection or electroporation
    • Help to protect your cells from toxicity or innate immune response activation

    Alt-R HDR Design Tool & Templates

    Streamline your homology-directed repair (HDR) research. Simply provide basic information about your target site, then use the HDR tool to design and visualize your desired edit within the sequence. The HDR Design Tool will provide the recommended gRNA(s) and HDR donor template for your specifications. 

    Alt-R HDR Donor Oligos

    Single-stranded DNA oligos specifically built for your homology-directed repair (HDR) experiments. Designed and optimized through extensive wet-bench testing, they are ideal for introducing point mutations and short insertions.

    Alt-R HDR Donor Blocks

    Double-stranded DNA fragments specifically built for your homology-directed repair (HDR) experiments. IDT dsDNA templates offer a cost-effective, high-fidelity option to reduce the amount of downstream screening via higher HDR rates and lower unintended blunt integrations. Alt-R HDR Donor Blocks are ideal for making large insertions or genomic changes.

    Alt-R CRISPR-Cas12a (Cpf1) System

    The Alt-R CRISPR-Cas12a System allows targeting of alternative sites that are not available to the CRISPR-Cas9 System and produces a staggered cut with a 5′ overhang.

    • Enables genome editing in organisms with AT-rich genomes
    • Allows interrogation of additional genomic regions compared to Cas9
    • Requires simple complexing of crRNA with the Cas12a protein—no tracrRNA needed

    Alt-R™ CRISPR Custom Guide RNAs

    Guide RNAs for novel Cas enzymes (e.g., prime editing or the Cas13 family)

    Our well-established expertise in oligo synthesis combined with an active CRISPR research and development program are what make us a trusted vendor for custom gRNA synthesis.

    rhAmpSeq CRISPR Analysis System

    The rhAmpSeq CRISPR Analysis System allows quick and accurate quantification of CRISPR-Cas edits. Our proprietary RNAse H2-dependent PCR technology generates amplicon libraries for targeted sequencing on Illumina® NGS platforms. The system also includes an advanced but accessible cloud-based data analysis pipeline for quantification of on- and off-target edits.

    https://www.idtdna.com/pages/products/crispr-genome-editing